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HomeMy WebLinkAbout7.12.22 Board Correspondence - FW_ CIMB _ Free Full-Text _ Intracellular Reverse Transcription of Pfizer BioNTech COVID-19 mRNA Vaccine BNT162b2 In Vitro in Human Liver Cell Line (2) From:Paulsen, Shaina To:Pickett, Andy; Reaster, Kayla; Valencia, Shyanne Subject:Board Correspondence - FW: CIMB | Free Full-Text | Intracellular Reverse Transcription of Pfizer BioNTech COVID-19 mRNA Vaccine BNT162b2 In Vitro in Human Liver Cell Line Date:Tuesday, July 12, 2022 9:13:54 AM Shaina Paulsen Associate Clerk of The Board Butte County Administration 25 County Center Drive, Suite 200, Oroville, CA 95965 T: 530.552.3304 | F: 530.538.7120 -----Original Message----- From: lance dreiss <lancedreiss@att.net> Sent: Friday, July 8, 2022 2:43 PM To: Ritter, Tami <TRitter@buttecounty.net>; District Attorney <District_Attorney@buttecounty.net>; balpert@buttecounty.net; Waugh, Melanie <mwaugh@buttecounty.net>; Kimmelshue, Tod <TKimmelshue@buttecounty.net>; Connelly, Bill <BConnelly@buttecounty.net>; Lucero, Debra <DLucero@buttecounty.net>; Teeter, Doug <DTeeter@buttecounty.net>; Clerk of the Board <clerkoftheboard@buttecounty.net>; Stephens, Brad J. <BStephens@buttecounty.net> Subject: CIMB | Free Full-Text | Intracellular Reverse Transcription of Pfizer BioNTech COVID-19 mRNA Vaccine BNT162b2 In Vitro in Human Liver Cell Line .ATTENTION: This message originated from outside Butte County. Please exercise judgment before opening attachments, clicking on links, or replying.. Board of Supervisors, Clerk of the Board, Public Record, DA Ramsey, Sheriff Honea, County Counsel: New lawsuits coming. Look up G.I.N.A. We reported this to Board of Supervisors a long time ago. Go back and view the videos. diana dreiss https://urldefense.com/v3/__https://www.mdpi.com/1467- 3045/44/3/73__;!!KNMwiTCp4spf!BDy432iltxtRn44UwogK-PmlUNpIqpRPLdTX6XfJjfmV1zvvyv5P- Ad7fARODj6Tmdeawsj6k8Id6LrsQIUuqB-uf-WgAQ$ Sent from my iPad CIMB | Free Full-Text | Intracellular Reverse Transcription of Pfizer BioNTech COVID-19 mRNA Vaccine BNT162b2 In Vitro in Human Liver Cell Line searchmenu Journals / CIMB / Volume 44 / Issue 3 / 10.3390/cimb44030073 / Submit to this Journal Review for this Journal Edit a Special Issue Article Menu error_outline Comment published on 11 April 2022, see Curr. Issues Mol. Biol. 2022, 44(4), 1661-1663. Open AccessArticle 1 1 1 1 by Markus Aldén,,,, 1 2 1,* , and 1 Department of Clinical Sciences, Lund University, 20502 Malmö, Sweden 2 Infection Medicine, Department of Clinical Sciences, Lund University, 22362 Lund, Sweden * Author to whom correspondence should be addressed. Academic Editor: Stephen Malnick Curr. Issues Mol. Biol. 2022, 44(3), 1115-1126; (This article belongs to the Topic ) 520523 1347677 cookies 14 Accept file:///C/...oNTech%20COVID-19%20mRNA%20Vaccine%20BNT162b2%20In%20Vitro%20in%20Human%20Liver%20Cell%20Line.html\[8/22/2022 2:34:34 PM\] CIMB | Free Full-Text | Intracellular Reverse Transcription of Pfizer BioNTech COVID-19 mRNA Vaccine BNT162b2 In Vitro in Human Liver Cell Line Altmetrics26464 keyboard_arrow_up Preclinical studies of COVID-19 mRNA vaccine BNT162b2, developed by Pfizer and BioNTech, showed reversible hepatic effects in animals that received the BNT162b2 injection. Furthermore, a recent study showed that SARS-CoV-2 RNA can be reverse- transcribed and integrated into the genome of human cells. In this study, we investigated the effect of BNT162b2 on the human liver cell line Huh7 in vitro. Huh7 cells were exposed to BNT162b2, and quantitative PCR was performed on RNA extracted from the cells. We detected high levels of BNT162b2 in Huh7 cells and changes in gene expression of long interspersed nuclear element-1 (LINE-1), which is an endogenous reverse transcriptase. Immunohistochemistry using antibody binding to LINE-1 open reading frame-1 RNA-binding protein (ORFp1) on Huh7 cells treated with BNT162b2 indicated increased nucleus distribution of LINE-1. PCR on genomic DNA of Huh7 cells exposed to BNT162b2 amplified the DNA sequence unique to BNT162b2. Our results indicate a fast up-take of BNT162b2 into human liver cell line Huh7, leading to changes in LINE-1 expression and distribution. We also show that BNT162b2 mRNA is reverse transcribed intracellularly into DNA in as fast as 6 h upon BNT162b2 exposure. Keywords: ; ; liver; reverse transcription; ; copyright This is an open access article distributed under the which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. keyboard_arrow_down file:///C/...oNTech%20COVID-19%20mRNA%20Vaccine%20BNT162b2%20In%20Vitro%20in%20Human%20Liver%20Cell%20Line.html\[8/22/2022 2:34:34 PM\] CIMB | Free Full-Text | Intracellular Reverse Transcription of Pfizer BioNTech COVID-19 mRNA Vaccine BNT162b2 In Vitro in Human Liver Cell Line SciFeed keyboard_arrow_down Article Metrics keyboard_arrow_down file:///C/...oNTech%20COVID-19%20mRNA%20Vaccine%20BNT162b2%20In%20Vitro%20in%20Human%20Liver%20Cell%20Line.html\[8/22/2022 2:34:34 PM\] CIMB | Free Full-Text | Intracellular Reverse Transcription of Pfizer BioNTech COVID-19 mRNA Vaccine BNT162b2 In Vitro in Human Liver Cell Line Related Articles keyboard_arrow_down Curr. Issues Mol. Biol., EISSN 1467-3045, Published by MDPI RSS Guidelines For Editors For Societies Sciforum Preprints Scilit SciProfiles journals Select options Tfmfdupqujpot Foufszpvsfnbjmbeesftt/// Tvctdsjcf © 1996-2022 MDPI (Basel, Switzerland) unless otherwise stated file:///C/...oNTech%20COVID-19%20mRNA%20Vaccine%20BNT162b2%20In%20Vitro%20in%20Human%20Liver%20Cell%20Line.html\[8/22/2022 2:34:34 PM\]