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05.27.25 Board Correspondence - FW_ Environmental and Recreational Compliance Report submitted in FERC P-619-000 by Pacific Gas and Electric Company
From:Clerk of the Board To:Clerk of the Board; Connelly, Bill; Cook, Holly; Cook, Robin; Durfee, Peter; Jessee, Meegan; Kimmelshue, Tod; Kitts, Melissa; Lee, Lewis; Little, Melissa; Pickett, Andy; Ritter, Tami; Stephens, Brad J.; Sweeney, Kathleen; Teeter, Doug; Zepeda, Elizabeth; Loeser, Kamie Cc:Nuzum, Danielle Subject:Board Correspondence - FW: Environmental and Recreational Compliance Report submitted in FERC P-619-000 by Pacific Gas and Electric Company Date:Tuesday, May 27, 2025 1:57:42 PM Please see Board Correspondence - -----Original Message----- From: 'FERC eSubscription' <eSubscription@ferc.gov> Sent: Tuesday, May 27, 2025 8:46 AM Subject: Environmental and Recreational Compliance Report submitted in FERC P-619-000 by Pacific Gas and Electric Company .ATTENTION: This message originated from outside Butte County. Please exercise judgment before opening attachments, clicking on links, or replying.. On 5/27/2025, the following Filing was submitted to the Federal Energy Regulatory Commission (FERC), Washington D.C.: Filer: Pacific Gas and Electric Company Docket(s): P-619-000 Lead Applicant: Pacific Gas and Electric Company Filing Type: Environmental and Recreational Compliance Report Description: Pacific Gas and Electric Company et al. submit 2024 Aquatic Invasive Species Plan Report re the Bucks Creek Hydroelectric Project under P-619. To view the document for this Filing, click here https://urldefense.com/v3/__https://elibrary.ferc.gov/eLibrary/filelist?accession_num=20250527- 5086__;!!KNMwiTCp4spf!DajiekV1003BsFP6pqNarFOst6TTYzDRGtOP_Wly_bc7an1UQeU07yKAuewYTr_gqGi2087m_cNpyvfenqzobA6WK2a1gs8irktD$ To modify your subscriptions, click here: https://urldefense.com/v3/__https://ferconline.ferc.gov/eSubscription.aspx__;!!KNMwiTCp4spf!DajiekV1003BsFP6pqNarFOst6TTYzDRGtOP_Wly_bc7an1UQeU07yKAuewYTr_gqGi2087m_cNpyvfenqzobA6WK2a1goXGmoRk$ ------------------------------------------------------------------------ Please do not respond to this email. Online help is available here: https://urldefense.com/v3/__http://www.ferc.gov/efiling-help.asp__;!!KNMwiTCp4spf!DajiekV1003BsFP6pqNarFOst6TTYzDRGtOP_Wly_bc7an1UQeU07yKAuewYTr_gqGi2087m_cNpyvfenqzobA6WK2a1gu4qAynU$ or for phone support, call 866-208-3676. Power Generation 300 Lakeside Drive Oakland, CA 94612 Mailing Address: P.O. Box 28209 Oakland, CA 94604 May 27, 2025 Via Electronic Submittal (E-File) Debbie-Anne Reese , Secretary Federal Energy Regulatory Commission Division of Hydropower Administration and Compliance 888 First Street, NE Washington, D.C. 20426 RE: Bucks Creek Hydroelectric Project, FERC No. 619-CA 2024 Aquatic Invasive Species Plan Report Dear Secretary Reese: This letter presents the 2024 Aquatic Invasive Species Monitoring Report (Report) for Pacific Gas and Electric Company’s (PG&E) Bucks Creek Hydroelectric Project, Federal Energy Regulatory Commission (FERC) No. 619. FERC issued a new license for the Bucks Creek Project on June 16, 2022. License Article 401(a) requires the licensees to implement resource protection plans required by State Water Resources Control Board (SWRCB) 401 Water Quality Certification (WQC) conditions and U.S. Department of Agriculture, Forest Service’s (“Forest Service”) section 4(e) conditions with the Commission. WQC Condition 17 and Forest Service section 4(e) Condition No. 44 require the Aquatic Invasive Species (AIS) Management Plan. In accordance with the approved AIS Plan, PG&E began implementing the Plan in License Year 1 (2023), the first full calendar year after license issuance. PG&E provided a Draft Aquatic Invasive Species Report to the Forest Service, SWRCB, California Department of Fish and Wildlife (CDFW), and United States Fish and Wildlife Service (USFWS) by email on February 4, 2025 . Over 60 days was allowed for review and comment. No comments were received. The final Report is provided in (Enclosure 1). Documentation of agency consultation is provided in (Enclosure 2). For questions, please contact Jamie Visinoni, senior license coordinator for PG&E, at (530) 215-6676. Sincerely, Megan Young License Project Manager Enclosures : 1. 2024 Aquatic Invasive Species Report , prepared by PG&E and dated 2025 2. Consultation Record, February 2025 cc: See Attached List Document Accession #: 20250527-5086 Filed Date: 05/27/2025 Debbie-Anne Reese, Secretary May 27, 2025 Page 2 cc: via email w/enclosures Bryan Muro (SWRCB) – Bryan.muro@Waterboards.ca.gov Nathan Fisch (SWRCB) – Nathan.fisch@waterboards.ca.gov Leslie Edlund (Forest Service) – Leslie.edlund@usda.gov Erika Brenzovich (Forest Service) – Erika.brenzovich@usda.gov Kurt Sable (Forest Service) – Kurt.sable@usda.gov Dawn Alvarez (Forest Service) - Dawn.alvarez@usda.gov Leigh Bartoo (USFWS) - Aondrea_Bartoo@fws.gov Michael Maher (CDFW) – Michael.Maher@wildlife.ca.gov Document Accession #: 20250527-5086 Filed Date: 05/27/2025 ENCLOSURE 1 Document Accession #: 20250527-5086 Filed Date: 05/27/2025 PACIFIC GAS AND ELECTRIC COMPANY AND THE CITY OF SANTA CLARA Bucks Creek Hydroelectric Project FERC Project No. 619 2024 Aquatic Invasive Species Monitoring Report – Final April 2025 ©2025, Pacific Gas and Electric Company and the City of Santa Clara Document Accession #: 20250527-5086 Filed Date: 05/27/2025 Bucks Creek Hydroelectric Project, FERC Project No. 619 2024 Aquatic Invasive Species Monitoring Report – Final This Page Intentionally Left Blank Document Accession #: 20250527-5086 Filed Date: 05/27/2025 PACIFIC GAS AND ELECTRIC COMPANY AND THE CITY OF SANTA CLARA Bucks Creek Hydroelectric Project FERC Project No. 619 2024 Aquatic Invasive Species Monitoring Report Final April 2025 ©2025, Pacific Gas and Electric Company and the City of Santa Clara Document Accession #: 20250527-5086 Filed Date: 05/27/2025 Bucks Creek Hydroelectric Project, FERC Project No. 619 2024 Aquatic Invasive Species Monitoring Report – Final This Page Intentionally Left Blank Document Accession #: 20250527-5086 Filed Date: 05/27/2025 Bucks Creek Hydroelectric Project, FERC Project No. 619 2024 Aquatic Invasive Species Monitoring Report – Final April 2025 Page i Contents Bucks Creek Hydroelectric Project, FERC Project No. 619 ©2025, Pacific Gas and Electric Company and the City of Santa Clara Bucks Creek Hydroelectric Project FERC Project No. 619 2024 Aquatic Invasive Species Monitoring Report TABLE OF CONTENTS SECTION 1.0 INTRODUCTION........................................................................................ 1-1 1.1 Background............................................................................................................... 1-1 1.2 Project Location and Feature Description ............................................................... 1-1 1.3 Purpose ..................................................................................................................... 1-2 1.4 Aquatic Invasive Species ......................................................................................... 1-2 SECTION 2.0 METHODS ................................................................................................... 2-1 2.1 Focal Species Monitoring ........................................................................................ 2-1 2.1.1 Quagga and Zebra (Dreissenid) Mussel Monitoring ............................... 2-1 2.1.2 Crayfish Monitoring................................................................................. 2-3 2.1.3 Other Focal AIS Monitoring .................................................................... 2-4 2.2 Incidental Observations ............................................................................................ 2-6 2.3 Data from online AIS databases .............................................................................. 2-7 SECTION 3.0 MONITORING RESULTS ......................................................................... 3-1 3.1 Focal Species Monitoring Results ........................................................................... 3-1 3.1.1 Quagga and Zebra (Dreissenid) Mussels ................................................. 3-1 3.1.2 Crayfish .................................................................................................... 3-1 3.1.3 Other Focal Species ................................................................................. 3-1 3.1.4 Incidental Observations ........................................................................... 3-1 3.1.5 Aquatic Invasive Species Database Review ............................................ 3-3 SECTION 4.0 DISCUSSION ............................................................................................... 4-1 4.1 Quagga and Zebra (Dreissenid) Mussels ................................................................. 4-1 4.2 Crayfish .................................................................................................................... 4-1 Document Accession #: 20250527-5086 Filed Date: 05/27/2025 Bucks Creek Hydroelectric Project, FERC Project No. 619 2024 Aquatic Invasive Species Monitoring Report – Final Contents Page ii April 2025 Bucks Creek Hydroelectric Project, FERC Project No. 619 ©2025, Pacific Gas and Electric Company and the City of Santa Clara 4.3 Other Focal Species .................................................................................................. 4-1 4.4 Variances from the Monitoring Plan ....................................................................... 4-1 SECTION 5.0 REFERENCES ................................................................................................ 1 Document Accession #: 20250527-5086 Filed Date: 05/27/2025 Bucks Creek Hydroelectric Project, FERC Project No. 619 2024 Aquatic Invasive Species Monitoring Report – Final April 2025 Page iii Contents Bucks Creek Hydroelectric Project, FERC Project No. 619 ©2025, Pacific Gas and Electric Company and the City of Santa Clara LIST OF ATTACHMENTS Attachment A Response to Comments on Agency Review Draft Document Accession #: 20250527-5086 Filed Date: 05/27/2025 Bucks Creek Hydroelectric Project, FERC Project No. 619 2024 Aquatic Invasive Species Monitoring Report – Final Contents Page iv April 2025 Bucks Creek Hydroelectric Project, FERC Project No. 619 ©2025, Pacific Gas and Electric Company and the City of Santa Clara LIST OF TABLES Table 1. Conditions of low, marginal, and high calcium (Ca) and pH that are Unable, Potentially Able, and Able to support dreissenid mussels (from Claudi and Prescott 2011), and vulnerability assessment/level of risk for waterbodies exhibiting described water quality characteristics. ....................... 2-2 Table 2. Risk-based dreissenid mussel monitoring schedule for Licensees’ lakes and reservoirs. ................................................................................................... 2-2 Table 3. Water quality parameters collected during quagga/zebra mussel monitoring. ........................................................................................................ 2-3 Table 4. Location of Bucks Creek Project (FERC Project No. 619) stream fish/crayfish monitoring sites, 2023. ................................................................. 2-3 Table 5. Location of Bucks Creek Project (FERC Project No. 619) benthic macroinvertebrate and monitoring sites and aquatic invasive species eDNA sample sites, 2023. ................................................................................. 2-5 Table 6. Locations of Bucks Creek Project (FERC Project No. 619) Sierra Nevada yellow-legged frog/aquatic invasive species eDNA monitoring sites. .................................................................................................................. 2-6 Table 7. Environmental DNA samples collected, and total volume filtered, and detection results for Sierra Nevada yellow-legged frog and aquatic invasive species at Sierra Nevada yellow-legged frog monitoring sites, Bucks Creek Project (FERC Project No. 619), 2023. ....................................... 3-2 Table 8. Non-indigenous species documented in the Milk Ranch Creek (including Three Lakes), Bucks Creek (including Bucks Lake and Lower Bucks Lake), and Grizzly Creek (including Grizzly Forebay) watersheds, Plumas Co., CA, from the USGS Non-Indigenous Aquatic Species (NAS) database, January 29, 2025. ................................................................... 3-4 Document Accession #: 20250527-5086 Filed Date: 05/27/2025 Bucks Creek Hydroelectric Project, FERC Project No. 619 2024 Aquatic Invasive Species Monitoring Report – Final April 2025 Page 1-1 Section 1.0 Bucks Creek Hydroelectric Project, FERC Project No. 619 ©2025, Pacific Gas and Electric Company and the City of Santa Clara SECTION 1.0 Introduction 1.1 Background Pacific Gas and Electric Company (PG&E) and the City of Santa Clara (City) received a new license for the Bucks Creek Hydroelectric Project (FERC Project No. 619) (Project) on June 16, 2022. The new license establishes the conditions under which the Project must be operated. Among those conditions is Article 401 which requires PG&E and the City to implement 13 management or monitoring plans, including the Aquatic Invasive Species Management Plan (Plan) which is also required by the Water Quality Certification Condition 17 and US Forest Service Condition 44. Elements of this plan were filed with PG&E’s Final License Application on December 12, 2016 pending further consultation with the stakeholders, and the final Plan was submitted to FERC in a supplemental filing on September 20, 2019. This plan was approved by FERC in the new license. In accordance with the approved Plan, PG&E began implementing the Plan in License Year 1 (2023), the first full calendar year after license issuance. This report and its attachments document the results of the 2024 monitoring. 1.2 Project Location and Feature Description The Project is located entirely within Plumas County, California in the Sierra Nevada Mountains, approximately 17 miles (27 kilometers [km]) southwest of the community of Quincy. The Project consists of two developments that combined contain four dams, four reservoirs, two powerhouses, and associated facilities. The Bucks Creek Development was completed in 1928 and includes Lower and Middle Three Lakes, Lower Bucks Lake, Bucks Lake, Grizzly Forebay, and Bucks Powerhouse. The Grizzly Development, which was completed in 1993, added the Grizzly Tunnel and Grizzly Powerhouse to the Project. Bucks Lake Dam is located on Bucks Creek and impounds Bucks Lake. From Bucks Lake, the Project’s water flow is released immediately downstream into Lower Bucks Lake, which is impounded by Lower Bucks Dam. Three Lakes Dam impounds the flow of Milk Ranch Creek at Middle Lake and Lower Lake. These water bodies, along with Upper Lake, are hydraulically connected and collectively known as Three Lakes Reservoir. Milk Ranch Conduit conveys the Project’s water flow from Three Lakes Reservoir and feeder diversions to Lower Bucks Lake. The Grizzly Powerhouse Tunnel conveys the water flow from Lower Bucks Lake to Grizzly Powerhouse. The powerhouse discharges the flow into Grizzly Forebay, which is impounded by Grizzly Forebay Dam. From Grizzly Forebay, the Project’s water flow is conveyed through the Grizzly Forebay Tunnel to Bucks Creek Powerhouse, which discharges the Project’s water flow into the North Fork Feather River (NFFR), upstream of Rock Creek Powerhouse, part of PG&E’s Rock Creek-Cresta Hydroelectric Project (FERC Project No. 1962). Document Accession #: 20250527-5086 Filed Date: 05/27/2025 Bucks Creek Hydroelectric Project, FERC Project No. 619 2024 Aquatic Invasive Species Monitoring Report – Final Section 1.0 Page 1-2 April 2025 Bucks Creek Hydroelectric Project, FERC Project No. 619 ©2025, Pacific Gas and Electric Company and the City of Santa Clara 1.3 Purpose This report documents the second year of AIS monitoring program (including incidental observations during coordinated monitoring efforts) under the new FERC license for the Bucks Creek Project. The monitoring program, in part, is intended to ensure early detection of AIS. Any incidental observations of AIS encountered during field surveys will also be documented in the report. 1.4 Aquatic Invasive Species The FERC-approved Bucks Creek Aquatic Invasive Species Management Plan included BMPs to prevent the introduction of AIS into Project-affected waters, and to provide early detection monitoring and monitoring for focal AIS populations. Focal AIS include species that occur or have the potential to be introduced into Project-affected waters within the Bucks Creek Project include: • Whirling Disease vector (Myxobolus cerebralis), • Ceratonova shasta, • Tetracapsuloides bryosalmonae, • Ichthyophthirius multifiliis, • Batrachochytrium dendrobatidis, • Ranavirus (Frog virus 3), • quagga mussels (Dreissena bugensis), • zebra mussels (D. polymorpha), • New Zealand mudsnail (Potamopyrgus antipodarum), • Asian clam (Corbicula fluminea), • signal crayfish (Pacifastacus leniusculus), • virile crayfish (Orconectes virilus), • American bullfrog (Lithobates catesbeianus), • curly leaf pondweed (Potamogeton crispus), • Eurasian milfoil (Myriophyllum spicatum), • Hydrilla (Hydrilla verticillata), • Brazilian waterweed (Egeria densa), • parrot’s feather milfoil (Myriophyllum aquaticum), • water primrose (Ludwigia spp.), and • didymo (Didymosphenia geminata). Document Accession #: 20250527-5086 Filed Date: 05/27/2025 Bucks Creek Hydroelectric Project, FERC Project No. 619 2024 Aquatic Invasive Species Monitoring Report – Final April 2025 Page 2-1 Section 2.0 Bucks Creek Hydroelectric Project, FERC Project No. 619 ©2025, Pacific Gas and Electric Company and the City of Santa Clara SECTION 2.0 Methods 2.1 Focal Species Monitoring The first year of AIS monitoring under the new license was conducted in 2023 and included quagga and zebra mussel monitoring consisting of surface surveys, a water quality profile, calcium sampling, and veliger tows. Water quality monitoring will determine the scope and frequency of future monitoring during the license term. Monitoring for crayfish and other focal species was implemented in parallel with other aquatic species monitoring for efficiency. In brief, the crayfish monitoring was conducted at the stream fish population monitoring sites and other focal species monitoring was implemented in parallel with benthic macroinvertebrate (BMI) and Sierra Nevada yellow-legged frog monitoring. 2.1.1 Quagga and Zebra (Dreissenid) Mussel Monitoring The type, location, and intensity of monitoring at each reservoir was determined in 2023 by the vulnerability assessment completed for the Project reservoirs (PG&E et al. 2019a). Using Project and local watershed data and the water quality thresholds for supporting dreissenid mussels examined by Claudi and Prescott (2011), the assessment will determine the vulnerability of Project reservoirs to infestation and monitoring schedule (Tables 1 and 2, respectively). Calcium samples were collected from the surface, mid-water, and bottom of each Project reservoir profile and sent to a laboratory for analysis (Table 3). Water quality profiles were collected in situ using a multiparameter probe (e.g., HYDROLAB) and include water temperature, dissolved oxygen, pH, turbidity, and conductivity (Table 3). The vulnerability to infestation for Project waterbodies is assessed as “High,” “Moderate,” “Low,” or “None” using water quality parameter thresholds (i.e., calcium concentration and pH) essential to supporting the survival of quagga and zebra mussels (Claudi and Prescott 2011). Based on this vulnerability, the scope and schedule of quagga and zebra (dreissenid) mussel monitoring was determined (Table 3-3). Monitoring may consist of surface surveys, artificial substrate monitoring, and/or plankton tow sampling utilizing the most current CDFW protocols (available online at https://www.wildlife.ca.gov/Conservation/Invasives/Quagga-Mussels). The monitoring schedule can vary from annual monthly monitoring June through September (High risk; Ca > 15 ppm [15 mg/L] and pH > 7.8) to every 10 years (risk rated as None; Ca ≤ 12 ppm [12 mg/L] and pH ≤ 7.3). Monitoring will be conducted at Project reservoirs where recreational, boating, or fishing activities are permitted, which includes Bucks Lake, Lower Bucks Lake, Grizzly Forebay, and Three Lakes. As appropriate, new water quality data for the Project will be reviewed as it becomes available. The vulnerability assessment may be updated using this information and/or any new scientific findings on the biological requirements and tolerances of quagga and zebra mussels. Any updates to the vulnerability assessment and quagga and zebra mussel monitoring updates will be provided in the annual AIS report to the Resources Agencies. Document Accession #: 20250527-5086 Filed Date: 05/27/2025 Bucks Creek Hydroelectric Project, FERC Project No. 619 2024 Aquatic Invasive Species Monitoring Report – Final Section 2.0 Page 2-2 April 2025 Bucks Creek Hydroelectric Project, FERC Project No. 619 ©2025, Pacific Gas and Electric Company and the City of Santa Clara Table 1. Conditions of low, marginal, and high calcium (Ca) and pH that are Unable, Potentially Able, and Able to support dreissenid mussels (from Claudi and Prescott 2011), and vulnerability assessment/level of risk for waterbodies exhibiting described water quality characteristics. pH Calcium Concentration Ca ≤ 12 mg/L 12 mg/L< Ca ≤ 15 mg/L Ca > 15 mg/L pH ≤ 7.3 Unable/ None Unable/ Low Unable/ Low 7.3 < pH ≤ 7.8 Unable/ Low Potentially Able/ Moderate Potentially Able/ Moderate pH > 7.8 Unable/ Low Potentially Able/ Moderate Able/ High Table 2. Risk-based dreissenid mussel monitoring schedule for Licensees’ lakes and reservoirs. Risk Parameters1 Periodicity Frequency Timing 1. High (Able to support dreissenid mussels) • Mussel monitoring − Surface − veliger − artificial substrate • WQ monitoring annual monthly • low elevation waters (<4,500 ft): Apr - Nov • high elevation waters (>4,500 ft): May- Oct 2. Moderate (Potentially Able to support dreissenid mussels) • Mussel monitoring − surface − veliger2 − artificial substrate • WQ monitoring every 2 years monthly • low elevation waters (<4,500 ft): Apr - Nov • high elevation waters (>4,500 ft): May - Oct 3. Low (Unable to support dreissenid mussels) • Mussel monitoring − surface − veliger2 • WQ monitoring every 5 years 2x per sample year (spring and fall) Jun or Jul and Sep or Oct 4. None (Unable to support dreissenid mussels) • Mussel monitoring − surface − veliger2 • WQ monitoring every 10 years 2x per sample year (spring and fall) Jun or Jul and Sep or Oct 1 Surface monitoring will include docks, mooring lines, buoys, boat ramps, shoreline concrete structures, woody debris, etc. 2 Veliger sample collection and analysis is no longer supported from waterbodies with calcium concentrations less than 15 mg/L (Attachment A). Document Accession #: 20250527-5086 Filed Date: 05/27/2025 Bucks Creek Hydroelectric Project, FERC Project No. 619 2024 Aquatic Invasive Species Monitoring Report – Final April 2025 Page 2-3 Section 2.0 Bucks Creek Hydroelectric Project, FERC Project No. 619 ©2025, Pacific Gas and Electric Company and the City of Santa Clara Table 3. Water quality parameters collected during quagga/zebra mussel monitoring. Constituent Method Minerals Calcium EPA 200.7 In-Situ Temperature (°C) HYDROLAB Specific Conductance (µmhos) HYDROLAB pH HYDROLAB Turbidity HYDROLAB Dissolved Oxygen (% saturation) HYDROLAB Dissolved Oxygen (mg/L) HYDROLAB 2.1.2 Crayfish Monitoring Crayfish monitoring in Project-affected streams was concurrent with the Aquatic Monitoring Plan’s Stream Fish Population Monitoring (see Bucks Creek Aquatic Resources Monitoring Plan for Stream Fish Population Monitoring schedule; PG&E et al. 2019b). Sample locations are shown in Table 4. Table 4. Location of Bucks Creek Project (FERC Project No. 619) stream fish/crayfish monitoring sites, 2023. Site ID Location Description Location Coordinates (decimal degrees) Upstream Downstream F-MRC-0.521 Milk Ranch Creek upstream of the NFFR confluence N/A N/A F-MRC-0.301 Milk Ranch Creek upstream of the NFFR confluence 39.958894, -121.274778 39.95951, -121.274299 F-BC-7.172 Bucks Creek downstream of Lower Bucks Lake Dam 39.903417, -121.229961 39.904215, -121.230802 F-BC-1.98 Bucks Creek accessed via Tobin Trail 39.91564, -121.299803 39.914103, -121.299950 F-GC-7.03 Grizzly Creek downstream of Grizzly Forebay Dam 39.888001, -121.295852 39.888372, -121.296392 F-GC-2.891 Grizzly Creek upstream of the Wildcat Creek confluence N/A N/A Notes: NFFR – North Fork Feather River N/A – Not applicable 1 Sites F-MRC-0.52 and F-GC-2.89 were not sampled in 2023 due to changing environmental conditions restricting access to the sites. Site F-MRC-0.52 was relocated to F-MRC-0.30. There was no alternative site located for site F-GC-2.89. 2 Site F-BC-7.17 was separated into two non-contiguous segments (upper segment and lower segment), consistent with the 2015 site boundaries, due to a deep pool and large logjam in the channel that prevented effective sampling. Document Accession #: 20250527-5086 Filed Date: 05/27/2025 Bucks Creek Hydroelectric Project, FERC Project No. 619 2024 Aquatic Invasive Species Monitoring Report – Final Section 2.0 Page 2-4 April 2025 Bucks Creek Hydroelectric Project, FERC Project No. 619 ©2025, Pacific Gas and Electric Company and the City of Santa Clara Any crayfish observed and/or captured will be identified to species and enumerated, where possible; a subsample of captured crayfish representative of the species will be photographed. 2.1.3 Other Focal AIS Monitoring Environmental DNA (eDNA) sampling for the following species was conducted within Project stream reaches concurrent with the Aquatic Monitoring Plan’s BMI Population Monitoring (PG&E et al. 2019b)(Table 5), and at tributaries to Project Bucks Lake and Grizzly Forebay concurrent with the Sierra Nevada Yellow-legged Frog Management Plan monitoring surveys (PG&E et al. 2019c)(Table 6) using available genetic assays: • Myxobolus cerebralis (whirling disease; Barry et al. 2021) • Ceratonova shasta (myxosoporean parasite; Hallett et al. 2006) • Tetracapsuloides bryosalmonae (proliferative kidney disease [or PKD]; Hutchins et al. 2017) • Ichthyophthirius multifiliis (white spot; Hu et al. 2022) • Batrachochytrium dendrobatidis (Bd or chytrid; Boyle et al. 2004) • Iridoviridae sp. (ranavirus or frog virus 3; Leung et al. 2017) To the extent possible, BMI monitoring sites were located at or near sites used during relicensing studies. Samples at SNYLF sites were collected from each stream at a suitable location within 330 ft (100 m) from the confluence with the Project reservoir. At each BMI and SNYLF monitoring site, biologists collected at least two eDNA samples (i.e., filters) from near the water’s surface and filtered at least 1 liter of water in accordance with guidelines in the literature. Biologists used 0.45-micrometer Millipore SterivexTM filters and a peristaltic pump to collect all eDNA samples in situ (Bergman et al. 2016, Blankenship and Schumer 2022). To prevent contamination, field personnel wore single-use latex gloves and eDNA samples were collected prior to entering the river channel and were collected first from downstream sites before moving upstream. Negative controls were sampled daily by filtering 1 liter of distilled water prior to sample collection. Laboratory personnel analyzed eDNA samples following best practices for eDNA extraction and created and analyzed an extraction negative and quantitative polymerase chain reaction (qPCR) negative with every extraction batch and qPCR plate (Miya et al. 2016). Samples were analyzed with an internal positive control that ensured samples were not inhibited (i.e., a negative result signified DNA was not detected and not an indication of a failed qPCR reaction). Document Accession #: 20250527-5086 Filed Date: 05/27/2025 Bucks Creek Hydroelectric Project, FERC Project No. 619 2024 Aquatic Invasive Species Monitoring Report – Final April 2025 Page 2-5 Section 2.0 Bucks Creek Hydroelectric Project, FERC Project No. 619 ©2025, Pacific Gas and Electric Company and the City of Santa Clara Table 5. Location of Bucks Creek Project (FERC Project No. 619) benthic macroinvertebrate and monitoring sites and aquatic invasive species eDNA sample sites, 2023. Site ID Location Coordinates (decimal degrees) Location Description License Year 1 (2023) Sample Collection Latitude Longitude Milk Ranch Creek BMI-MRC-3.4 39.967318 -121.227413 Milk Ranch Creek downstream of Three Lakes Dam AIS eDNA BMI-MRC-0.12 39.961313 -121.273472 Milk Ranch Creek upstream of the NFFR confluence AIS eDNA Bucks Creek BMI-BC-6.9 39.904565 -121.233341 Bucks Creek downstream of Lower Bucks Lake Dam AIS eDNA BMI-BC-1.9 39.914006 -121.301389 Bucks Creek near the Tobin Trail BMI survey and AIS eDNA Grizzly Creek BMI-GC-7.1 39.888841 -121.294505 Grizzly Creek downstream of Grizzly Forebay Dam AIS eDNA BMI-GC-2.9 39.856137 -121.33499 Grizzly Creek upstream of the confluence with Wildcat Creek None1 Notes: AIS – aquatic invasive species BMI – benthic macroinvertebrates eDNA – environmental deoxyribonucleic acid NFFR – North Fork Feather River 1 AIS eDNA samples could not be collected during License Year 1 because the site was not safely accessible. Document Accession #: 20250527-5086 Filed Date: 05/27/2025 Bucks Creek Hydroelectric Project, FERC Project No. 619 2024 Aquatic Invasive Species Monitoring Report – Final Section 2.0 Page 2-6 April 2025 Bucks Creek Hydroelectric Project, FERC Project No. 619 ©2025, Pacific Gas and Electric Company and the City of Santa Clara Table 6. Locations of Bucks Creek Project (FERC Project No. 619) Sierra Nevada yellow-legged frog/aquatic invasive species eDNA monitoring sites. General Area Site ID Location Description Location Coordinates (decimal degrees) Upstream Downstream Three Lakes Road BR-11 Bear Ravine downstream of diversion 39.91579, -121.227309 39.915273, -121.231287 BR-21 Bear Ravine upstream of diversion 39.91829, -121.22136 39.915779, -121.225963 SFGHC-1 South Fork Grouse Hollow Creek downstream and upstream of diversion 39.958996, -121.234879 39.962776, -121.239515 Grizzly Creek GC-3 Grizzly Creek downstream of Grizzly Forebay Dam 39.889801, -121.294717 39.887994, -121.296287 GC-42 Grizzly Creek upstream of Grizzly Forebay 39.890144, -121.272816 39.88883, -121.279175 Tributaries to Bucks Lake HC-11,2 Haskins Creek at confluence with Bucks Lake 39.868135, -121.168189 39.86945, -121.175777 BC-21 Bucks Creek at confluence with Bucks Lake 39.886062, -121.146412 39.88156, -121.154139 RBMC-11,2 Right Hand Branch Mill Creek at confluence with Bucks Lake 39.900537, -121.167939 39.899613, -121.174128 MFMC-11,2 Middle Fork Mill Creek at confluence with Bucks Lake 39.906854, -121.175114 39.903325, -121.177546 MC-11,2 Mill Creek at confluence with Bucks Lake 39.919727, -121.185821 39.91521, -121.184683 1 Site is within Sierra Nevada yellow-legged frog critical habitat. 2 Aquatic invasive species required monitoring site. 2.2 Incidental Observations In addition to this focal species monitoring, PG&E was to report incidental observations of other AIS that could potentially occur in the project area while conducting other work, such as periodic aquatic surveys (i.e., fish, amphibian, BMI). These species include: • New Zealand mudsnail (Potamopyrgus antipodarum), • Asian clam (Corbicula fluminea), • American bullfrog (Lithobates catesbeianus), • curly leaf pondweed (Potamogeton crispus), • Eurasian milfoil (Myriophyllum spicatum), • Hydrilla (Hydrilla verticillata), Document Accession #: 20250527-5086 Filed Date: 05/27/2025 Bucks Creek Hydroelectric Project, FERC Project No. 619 2024 Aquatic Invasive Species Monitoring Report – Final April 2025 Page 2-7 Section 2.0 Bucks Creek Hydroelectric Project, FERC Project No. 619 ©2025, Pacific Gas and Electric Company and the City of Santa Clara • Brazilian waterweed (Egeria densa), • parrot’s feather milfoil (Myriophyllum aquaticum), • water primrose (Ludwigia spp.), and • didymo (Didymosphenia geminata). 2.3 Data from online AIS databases PG&E accessed and download data from online AIS databases, such as the USGS Non-Indigenous Aquatic Species (NAS) database (https://nas.er.usgs.gov/) and iMapInvasives (http://www.imapinvasives.org/vision) to determine if new AIS have been found in the Project Area. Document Accession #: 20250527-5086 Filed Date: 05/27/2025 Bucks Creek Hydroelectric Project, FERC Project No. 619 2024 Aquatic Invasive Species Monitoring Report – Final Section 2.0 Page 2-8 April 2025 Bucks Creek Hydroelectric Project, FERC Project No. 619 ©2025, Pacific Gas and Electric Company and the City of Santa Clara This Page Intentionally Left Blank Document Accession #: 20250527-5086 Filed Date: 05/27/2025 Bucks Creek Hydroelectric Project, FERC Project No. 619 2024 Aquatic Invasive Species Monitoring Report – Final April 2025 Page 3-1 Section 3.0 Bucks Creek Hydroelectric Project, FERC Project No. 619 ©2025, Pacific Gas and Electric Company and the City of Santa Clara SECTION 3.0 Monitoring Results 3.1 Focal Species Monitoring Results 3.1.1 Quagga and Zebra (Dreissenid) Mussels Based on the results of 2023 monitoring and the criteria in the Plan, it was determined that the three project reservoirs were unable to support quagga and zebra mussels and that sampling would be repeated every 5 years (PG&E 2024). The next sampling for these species will occur in License Year 6, 2028. 3.1.2 Crayfish Fish population surveys were completed at two sites (Bucks Creek site F-BC-7.17 and Grizzly Creek F-GC-7.03) on September 25 and 26, 2024. No crayfish were observed at either site. 3.1.3 Other Focal Species BMI sampling was not required in License Year 2, and SNYLF sampling was only required at two sites on Bear Ravine above and below the diversion. eDNA samples were collected at these two sites on August 27, 2024. eDNA for Batrachochytrium dendrobatidis (Bd) was detected one of four replicate samples at the site above the diversion. No eDNA for any other target AIS was detected in any of the samples (Table 11). During laboratory analysis, none of the samples showed signs of inhibition and all the negative control samples (including extraction negative and qPCR negative) tested negative. 3.1.4 Incidental Observations Didymosphenia geminata (commonly referred to as “didymo” or “rock snot”) was again observed at Site F-BC-7.17 on Bucks Creek below Lower Bucks Lake but was much less abundant than in 2023. It was not observed at F-GC-7.03 on Grizzly Creek. It was not observed here in 2023 but was detected in eDNA samples in 2023. No eDNA samples were collected in 2024. Document Accession #: 20250527-5086 Filed Date: 05/27/2025 Bucks Creek Hydroelectric Project, FERC Project No. 619 2024 Aquatic Invasive Species Monitoring Report – Final Section 5.0 Page 5-3-2 April 2025 Bucks Creek Hydroelectric Project, FERC Project No. 619 ©2025, Pacific Gas and Electric Company and the City of Santa Clara Table 7. Environmental DNA samples collected, and total volume filtered, and detection results for Sierra Nevada yellow-legged frog and aquatic invasive species at Sierra Nevada yellow-legged frog monitoring sites, Bucks Creek Project (FERC Project No. 619), 2023. Site ID eDNA Samples Collected (n) Total Volume Filtered (liter) eDNA detected (yes/no) Ra n a s i e r r a e (S i e r r a N e v a d a ye l l o w -le g g e d f r o g ) Di d y m o s p h e n i a g e m i n a t e (D i d y m o ) My x o b o l u s c e r e b r a l i s (w h i r l i n g d i s e a s e ) Ce r a t o n o v a sh a s t a (m y x o s o p o r e a n p a r a s i t e ) Te t r a c a p s u l o i d e s br y o s a l m o n a e (p r o l i f e r a t i v e k i d n e y di s e a s e ) Ic h t h y o p h t h i r i u s m u l t i f i l i i s (w h i t e s p o t ) Ba t r a c h o c h y t r i u m de n d r o b a t i d i s (B d ) Ir i d o v i r d a e sp . (r a n a v i r u s ) BR-1 4 4 No No No No No No Yes1 No BR-2 4 4 Yes No No No No No No No Notes: *AIS required eDNA monitoring site eDNA – environmental deoxyribonucleic acid n – number L – Liter PKD – proliferative kidney disease SNYLF – Sierra Nevada yellow-legged frog 1 Detected in one technical replicate in one sample (of four total) replicates collected at this location. See text. Document Accession #: 20250527-5086 Filed Date: 05/27/2025 Bucks Creek Hydroelectric Project, FERC Project No. 619 2024 Aquatic Invasive Species Monitoring Report – Final April 2025 Page 3-3 Section 3.0 Bucks Creek Hydroelectric Project, FERC Project No. 619 ©2025, Pacific Gas and Electric Company and the City of Santa Clara 3.1.5 Aquatic Invasive Species Database Review To the extent available, aquatic invasive species databases were queried by Watershed Hydrologic Unit Code (HUC): • Milk Ranch Creek (including Three Lakes), HUC 12-180201210603 • Bucks Creek (including Bucks Lake and Lower Bucks Lake), HUC 12-180201210602 • Grizzly Creek (including Grizzly Forebay), HUC 12-180201210605 The USGS Non-Indigenous Aquatic Species (NAS) database (https://nas.er.usgs.gov/) was queried by creating polygons of the Milk Ranch Creek (including Three Lakes), Bucks Creek (including Bucks Lake and Lower Bucks Lake), and Grizzly Creek (including Grizzly Forebay) watersheds. The results of last year’s query identified rainbow trout, Lahontan redside, brook trout, and lake trout as non-indigenous species are provided in Table 13 (USGS 2024). This year’s search found the same species with the addition of Didymo, which was identified during last year’s field surveys and reported to the database (USGS 2025). No relevant information could be queried from iMapInvasives (http://www.imapinvasives.org/vision), as the site does have support (data) from California. Document Accession #: 20250527-5086 Filed Date: 05/27/2025 Bucks Creek Hydroelectric Project, FERC Project No. 619 2024 Aquatic Invasive Species Monitoring Report – Final Section 5.0 Page 5-3-4 April 2025 Bucks Creek Hydroelectric Project, FERC Project No. 619 ©2025, Pacific Gas and Electric Company and the City of Santa Clara Table 8. Non-indigenous species documented in the Milk Ranch Creek (including Three Lakes), Bucks Creek (including Bucks Lake and Lower Bucks Lake), and Grizzly Creek (including Grizzly Forebay) watersheds, Plumas Co., CA, from the USGS Non-Indigenous Aquatic Species (NAS) database, January 29, 2025. Specimen Number Species ID Group Family Scientific Name Common Name Latitude/ Longitude Accuracy Status 1724810 2856 Algae Gomphone- mataceae Didymosphenia geminata didymo 39.9045/ -121.231 Accurate 167680 910 Fishes Salmonidae Oncorhynchus mykiss Rainbow Trout 39.88655/ -121.191 Accurate established 28852 647 Fishes Cyprinidae Richardsonius egregius Lahontan Redside 39.88655/ -121.191 Accurate established 167681 939 Fishes Salmonidae Salvelinus fontinalis Brook Trout 39.88655/ -121.191 Accurate established 167682 942 Fishes Salmonidae Salvelinus namaycush Lake Trout 39.88655/ -121.191 Accurate established Document Accession #: 20250527-5086 Filed Date: 05/27/2025 Bucks Creek Hydroelectric Project, FERC Project No. 619 2024 Aquatic Invasive Species Monitoring Report – Final April 2025 Page 4-1 Section 4.0 Bucks Creek Hydroelectric Project, FERC Project No. 619 ©2025, Pacific Gas and Electric Company and the City of Santa Clara SECTION 4.0 Discussion 4.1 Quagga and Zebra (Dreissenid) Mussels Per the results of last year’s sampling and in accordance with the Plan, sampling was not conducted for dreissenid mussels in 2024. Sampling for dreissenid mussels will resume in 2028. 4.2 Crayfish No crayfish were observed during fish or Sierra Nevada yellow-legged frog monitoring in 2024. Unidentified crayfish were observed at a couple locations during the 2023 SNYLF and fish population monitoring. Crayfish monitoring will continue concurrent with the remaining SNYLF and stream fish population schedule. 4.3 Other Focal Species Didymo was observed again in Bucks Creek in 2024 but was less abundant than in 2023. It was not detected in eDNA samples collected in Bear Ravine as part of Sierra Nevada yellow-legged frog sampling. Batrachochytrium dendrobatidis was detected in one of four eDNA replicates collected at the upper site in Bear Ravine but was not detected in the eDNA replicates collected at the lower site. There were no positive detections for Myxobolus cerebralis, Ceratonova shasta, Tetracapsuloides bryosalmonae, Ichthyophthirius multifiliis, or Ranavirus (Frog virus 3) in the Sierra Nevada yellow-legged frog eDNA sampling in 2023. There is no known way to control or eradicate didymo once it is established in a watershed. PG&E has posted CDFW’s AIS posters and a Didymo specific poster at all campgrounds, public kiosks, and boat launches consistent with discussions with the agencies last year. PG&E requires that its employees and contractor personnel follow CDFW decontamination procedures when moving between water bodies To ensure early detection for AIS in the future, eDNA sampling for the aforementioned species will continue to be conducted at the BMI and SNYLF monitoring sites concurrent with the monitoring schedules for those programs (PG&E and City of Santa Clara 2019b, 2019c). 4.4 Variances from the Monitoring Plan There were no variances from the AIS Monitoring Plan in 2024. Document Accession #: 20250527-5086 Filed Date: 05/27/2025 Document Accession #: 20250527-5086 Filed Date: 05/27/2025 Bucks Creek Hydroelectric Project, FERC Project No. 619 2024 Aquatic Invasive Species Monitoring Report – Final April 2025 Page 5-1 Section 5.0 Bucks Creek Hydroelectric Project, FERC Project No. 619 ©2025, Pacific Gas and Electric Company and the City of Santa Clara SECTION 5.0 References Barry, D.E., M. Veillard, C.T. James, L. Brummelhuis, E.A. Pila, A. Turnbull, A. Oddy-van Oploo, X. Han, and P.C. Hanington. 2021. qPCR-based environmental monitoring of Myxobolus cerebralis and phylogenetic analysis of its tubificid hosts in Alberta, Canada. Diseases of Aquatic Organisms 145: 119-137. Bedwell, M.E. and C.S. Goldberg. 2020. Spatial and temporal patterns of environmental DNA detection to inform sampling protocols in lentic and lotic systems. Ecology and Evolution 10(3): 1602–1612 Bergman, P.S., G. Schumer, S. Blankenship, E. Campbell. 2016. Detection of Adult Green Sturgeon Using Environmental DNA Analysis. PLOS ONE. Apr 20;114:e0153500. Blankenship, S., and G. Schumer. 2022. Field collection procedure for aquatic environmental DNA sample collection and analysis. Cramer Fish Sciences - Genidaqs, West Sacramento, California. Boyle, D.G., D.B. Boyle, V. Olsen, J.A.T. Morgan, and A.D. Hyatt. 2004. Rapid quantitative detection of chytridiomycosis (Batrachochytrium dendrobatidis) in amphibian samples using real-time Taqman PCR assay. Diseases of Aquatic Organisms 60(2): 141–148. Claudi, R. and K. Prescott. 2011. Examination of Calcium and pH as predictors of Dreissenid Mussel Survival in the California State Water Project. Prepared for the California Department of Water Resources, Division of Operations and Maintenance, Aquatic Nuisance Species Program. Diggles, M.F., J.R. Rytuba, B.C. Moring, C.T. Wrucke, D.P. Cox, S. Ludington, R.P. Ashley, W.J. Pickthorn, C.T. Hillman, and R.J. Miller. 1996. Geology and Minerals Issues in Sierra Nevada Ecosystem Project: Final report to Congress, vol. II, Assessments and scientific basis for management options. Davis: University of California, Centers for Water and Wildland Resources, 1996. Hallett, S.L. and J.L. Bartholomew. 2006. Application of a real-time PCR assay to detect and quantify the myxozoan parasite Ceratomyxa shasta in river water samples. Diseases of Aquatic Organisms 71: 109-118. Document Accession #: 20250527-5086 Filed Date: 05/27/2025 Bucks Creek Hydroelectric Project, FERC Project No. 619 Aquatic Invasive Species Management Plan – Final Section 5.0 Page 5-2 April 2025 Bucks Creek Hydroelectric Project, FERC Project No. 619 ©2025, Pacific Gas and Electric Company and the City of Santa Clara Hu, G., K. Huang, W. Zhou, R. Wang, W. Zhao, H. Zou, W. Li, S. Wu, M. Li, and G. Wang. 2022. Comparison of droplet digital PCR and real-time quantitative PCR for quantitative detection of the parasitic ciliate Ichthyophthirius multifiliis in the water environment. Journal of Fish Diseases 46: 357-367. Hutchins, P.R., A.J. Sepulveda, R.M. Martin, and L.R. Hopper. 2017. A probe-based quantitative PCR assay for detecting Tetracapsuloides bryosalmonae in fish tissue and environmental DNA water samples. Conservation Genetics Resource 10: 317-319. Hutchins, P.R., A.J. Sepulveda, R.M. Martin, and L.R. Hopper. 2017. A probe-based quantitative PCR assay for detecting Tetracapsuloides bryosalmonae in fish tissue and environmental DNA water samples. Conservation Genetics Resource 10: 317-319. Leung, W.T.M., L. Thomas-Walters, T.W.J. Garner, F. Balloux, C. Durrant, and S.J. Price. 2017. A quantitative-PCR based method to estimate ranavirus viral load following normalisation by reference to an ultraconserved vertebrate target. Journal of Virological Methods 249: 147-155. Miya, M., T. Minamoto, H. Yamanaka, S.I. Oka, K. Sato, S. Yamamoto, T. Sado, and H. Doi. 2016. Use of a filter cartridge for filtration of water samples and extraction of environmental DNA. Journal of Visualized Experiments 117, p.e54741. PG&E (Pacific Gas and Electric) and the City of Santa Clara. 2016. Final License Application. Bucks Creek Hydroelectric Project, FERC Project No. 619. December. PG&E and the City of Santa Clara. 2019a. Aquatic Invasive Species Management Plan. Bucks Creek Hydroelectric Project, FERC Project No. 619. September. PG&E and City of Santa Clara. 2019b. Bucks Creek Hydroelectric Project (FERC Project No. 619) Aquatic Resources Monitoring Plan. September. PG&E and the City of Santa Clara. 2019c. Sierra Nevada Yellow-legged Frog Management Plan. Bucks Creek Hydroelectric Project, FERC Project No. 619. September. United States Geological Survey (USGS). 2024. Nonindigenous Aquatic Species point map viewer. Available online: https://nas.er.usgs.gov/ Accessed February 6, 2024. United States Geological Survey (USGS). 2025. Nonindigenous Aquatic Species point map viewer. Available online: https://nas.er.usgs.gov/ Accessed January 29, 2025. Document Accession #: 20250527-5086 Filed Date: 05/27/2025 Bucks Creek Hydroelectric Project, FERC Project No. 619 2024 Aquatic Invasive Species Monitoring Report – Final April 2025 Attachment A Bucks Creek Hydroelectric Project, FERC Project No. 619 ©2025, Pacific Gas and Electric Company and the City of Santa Clara Attachment A Response to Comments on Agency Review Draft No comments were received on the 2024 AIS Plan Document Accession #: 20250527-5086 Filed Date: 05/27/2025 ENCLOSURE 2 Document Accession #: 20250527-5086 Filed Date: 05/27/2025 From:Visinoni, Jamie To:dave@americanwhitewater.org; theresa@americanwhitewater.org; bigwolf_1962@yahoo.com; Michael.maher@wildlife.ca.gov; beth.lawson@wildlife.ca.gov; Omyepi1@gmail.com; aondrea_bartoo@fws.gov; trina@maidusummit.org; shannon@maidusummit.org; Muro, Bryan@Waterboards; Nathan.Fisch@waterboards.ca.gov; blancapaloma@msn.com; Valentina Guzman; NVanHaeften@santaClaraCA.gov; Hamilton, David - FS, CA; Mosinski, Kelly - FS, CA; cindy@cindycharles.net Cc:kurt.sable@usda.gov; Alvarez, Dawn -FS; Edlund, Leslie - FS; Brenzovich, Erika -FS; Wise Jr., Larry; Berry, Jonathan - FS, CA; Fiel, Andrew - FS, CA; ashlee.taylor@usda.gov; richard.hopson@usda.gov; caitlin.ostomel@usda.gov; Young, Megan; Stevens, Kevin; Fransz, Matthew Subject:Bucks Creek Hydroelectric Project, FERC Project No. 619 - 2024 Aquatic Invasive Species Monitoring Report Date:Tuesday, February 04, 2025 9:54:00 AM Attachments:image001.png Bucks Creek AIS Monitoring_2024_Agency draft.pdf Hello ECG and Resource Agencies, This email presents the Draft 2024 Aquatic Invasive Species Monitoring Report for Pacific Gas and Electric Company’s (PG&E) Bucks Creek Hydroelectric Project, Federal Energy Regulatory Commission (FERC) No. 619. FERC issued a new license for the Bucks Creek Project on June 16, 2022. License Article 401(a) requires the licensees to implement resource protection plans required by State Water Resources Control Board (SWRCB) 401 Water Quality Certification (WQC) conditions and U.S. Department of Agriculture, Forest Service’s (“Forest Service”) section 4(e) conditions with the Commission. WQC Condition 17 and Forest Service section 4(e) Condition No. 44 require the Aquatic Invasive Species (AIS) Management Plan. In accordance with the approved AIS Plan, PG&E began implementing the Plan in License Year 1 (2023), the first full calendar year after license issuance. The attached draft 2024 Aquatic Invasive Species Monitoring Report documents the results of the 2024 monitoring. Please provide any input on the draft report by March 6, 2025 (30 days from today) so that we are prepared to discuss any comments during the annual consultation meetings. Thank you, Jamie Visinoni Hydro License Coordinator | Power Generation m: (530) 215-6676 | e: jnvs@pge.com Document Accession #: 20250527-5086 Filed Date: 05/27/2025 Document Content(s) PGE20250527_619_Bucks_2024AIS_Plan_Report.pdf .............................1Document Accession #: 20250527-5086 Filed Date: 05/27/2025